Reovirus progeny subviral particles synthesize uncapped mRNA
نویسندگان
چکیده
منابع مشابه
Transcription by infectious subviral particles of reovirus.
Digestion of purified reovirus with chymotrypsin in the presence of 0.15 M NaCl converts virions to infectious subviral particles (SVP(i)). The SVP(i) have an active ribonucleic acid (RNA) polymerase and are similar in composition to the partially uncoated virions which have been isolated from infected L cells. SVP(i) have a buoyant density of 1.40 g/ml in CsCl and sediment at 420S as compared ...
متن کاملProperties of RNA transcriptase in reovirus subviral particles.
Subviral particles containing reovirus RNA transcriptase have been isolated from extracts of virus-infected mouse fibroblast cells. The purified particles which lacked the outer protein capsomeres of the mature virion had a buoyant density of 1.43-1.44 g/ml in CsCl and contained all of the double-stranded RNA genome of the intact virus. The particles were free of nuclease activity. RNA synthesi...
متن کاملFormation and characteristics of reovirus subviral particles in interferon-treated mouse L cells.
We examined the early phase of reovirus replication in interferon-treated L cells. No difference was detected in the adsorption of virus to cells, the rate and extent of conversion of parental virions to sub-viral particles (SVPs), and the protein and double-stranded RNA composition of the SVPs when comparing reovirus infection of the interferon-treated and control cells. Furthermore, when test...
متن کاملGenome-wide profiling of uncapped mRNA.
Gene transcripts are under extensive posttranscriptional regulation, including the regulation of their stability. A major route for mRNA degradation produces uncapped mRNAs, which can be generated by decapping enzymes, endonucleases, and small RNAs. Profiling uncapped mRNA molecules is important for the understanding of the transcriptome, whose composition is determined by a balance between mRN...
متن کاملRNA binding properties of poliovirus subviral particles.
The mechanism of encapsidation of the RNA genome of poliovirus and other picornaviruses is unknown. To test whether any of the putative assembly intermediates of poliovirus could interact directly with the poliovirus RNA genome, poliovirus RNA was attached to magnetic streptavidin beads and incubated with partially purified extracts containing 35S-labeled 14S pentamer and 75S empty-capsid subvi...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Virology
سال: 1980
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.34.2.497-505.1980